Journal: Journal of Anatomy

Article Title: Callosal septa express guidance cues and are paramedian guideposts for human corpus callosum development

doi: 10.1111/joa.13011

Figure Lengend Snippet: Primary antibodies used in the study

Article Snippet: Qualitative analysis of the stained histological sections was performed using an upright Olympus Provis AX70 microscope, and images were captured with a Nikon DXM1200 digital camera. table ft1 table-wrap mode="anchored" t5 Table 2 caption a7 Primary antibodies (clone) Name or abbreviation (Cat. No.) Host, isotype Dilution Supplier Anti‐Microtubule‐Associated Protein‐2 (HM2) MAP2 (M4403) Mouse, monoclonal, IgG1, affinity purified hybridoma cell culture 1:1000 Sigma‐Aldrich, St. Louis, MO, USA Anti‐Microtubule‐Associated Protein‐1B (clone AA6) MAP1B (M4528) Mouse, monoclonal, IgG1, affinity purified hybridoma cell culture 1:1000 Sigma‐Aldrich, St. Louis, MO, USA Anti‐synaptophysin (SY38) synaptophysin (M0776) Mouse, monoclonal IgG1 kappa, cell culture supernatant 1:100 Dako, Glostrup, Denmark, EU Anti‐ synaptosomal‐associated protein 25 (SMI81) SNAP25 (836301) Mouse monoclonal IgG1, kappa, affinity purified hybridoma cell culture 1: 1000 Biolegend, San Diego, CA, USA Growth associated protein ‐43 GAP43 (NB300‐143) Rabbit polyclonal affinity purified IgG 1:1000 Novus Biologicals, LLC,Centennial, CO, USA Anti‐neogenin (N‐19) neogenin (sc‐6537) Goat polyclonal affinity purified IgG 1:200 Santa Cruz, Biotechnology, Inc. Dallas, TX, USA Anti‐ephrinA4 (P‐20) ephrinA4 (sc‐912) Rabbit polyclonal affinited purified IgG 1:200 Santa Cruz, Biotechnology, Inc. Dallas, TX, USA Anti‐human fibronectin fibronectin (F3648) Rabbit polyclonal, affinity purified antibody 1:400 Sigma‐Aldrich, St. Louis, MO, USA Anti‐Glial Fibrillary Acidic Protein GFAP (Z0334) Rabbit, polyclonal purified immunoglobulin 1:1000 Dako Glostrup, Denmark, EU Anti‐human CD68 (514H12) CD68 (MCA1815) Mouse, monoclonal IgG2a, tissue Culture Supernatant 1:80 Bio‐Rad Antibodies Kidlington, Oxford, UK Open in a separate window Primary antibodies used in the study Nissl‐stained sections at the level of the roof of the lateral ventricle were used to count the callosal septa.

Techniques: Affinity Purification, Cell Culture, Purification